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17.06.2022 | Original Article

Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats

verfasst von: Aristides Tadeu Correia, Francine Maria de Almeida, Marcia Cristina Augusto-Cottet, Patrícia Nolasco, Afonso Silva Alves Bento, Hugo Kenji Matsushima Hirano, Maria Cecília Ribeiro de Souza, Elizabete Silva dos Santos, Júlia Helena Rodrigues de Castro, Monique Matsuda, Paulo Manuel Pêgo-Fernandes, Rogerio Pazetti

Erschienen in: Inflammation | Ausgabe 6/2022

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Abstract

Previous studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (p < 0.05). Neutral mucin percentage was higher in Triple group (p < 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (p < 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (p < 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (p < 0.05). The number of polymorphonuclear cells was higher in the Triple group (p < 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (p < 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (p < 0.05). BSX did not change the mucociliary apparatus of rats.

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Titel: Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats
verfasst von: Aristides Tadeu Correia
Francine Maria de Almeida
Marcia Cristina Augusto-Cottet
Patrícia Nolasco
Afonso Silva Alves Bento
Hugo Kenji Matsushima Hirano
Maria Cecília Ribeiro de Souza
Elizabete Silva dos Santos
Júlia Helena Rodrigues de Castro
Monique Matsuda
Paulo Manuel Pêgo-Fernandes
Rogerio Pazetti
Publikationsdatum: 17.06.2022
Verlag: Springer US
Erschienen in: Inflammation / Ausgabe 6/2022
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-022-01687-0

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