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01.12.2023 | Original Article

Diagnosing COVID-19 in nasopharyngeal secretion through Raman spectroscopy: a feasibility study

verfasst von: Ana Cristina Castro Goulart, Renato Amaro Zângaro, Henrique Cunha Carvalho, Igor K. Lednev, Landulfo Silveira Jr

Erschienen in: Lasers in Medical Science | Ausgabe 1/2023

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Abstract

Since the beginning of the COVID-19 pandemic, the scientific community has sought to develop fast and accurate techniques for detecting the SARS-CoV-2 virus. Raman spectroscopy is a promising technique for diagnosing COVID-19 through serum samples. In the present study, the diagnosis of COVID-19 through nasopharyngeal secretion has been proposed. Raman spectra from nasopharyngeal secretion samples (15 Control, negative and 12 COVID-19, positive, assayed by immunofluorescence antigen test) were obtained in triplicate in a dispersive Raman spectrometer (830 nm, 350 mW), accounting for a total of 80 spectra. Using principal component analysis (PCA) the main spectral differences between the Control and COVID-19 samples were attributed to N and S proteins from the virus in the COVID-19 group. Features assigned to mucin (serine, threonine and proline amino acids) were observed in the Control group. A binary model based on partial least squares discriminant analysis (PLS-DA) differentiated COVID-19 versus Control samples with accuracy of 91%, sensitivity of 80% and specificity of 100%. Raman spectroscopy has a great potential for becoming a technique of choice for rapid and label-free evaluation of nasopharyngeal secretion for COVID-19 diagnosis.

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Titel: Diagnosing COVID-19 in nasopharyngeal secretion through Raman spectroscopy: a feasibility study
verfasst von: Ana Cristina Castro Goulart
Renato Amaro Zângaro
Henrique Cunha Carvalho
Igor K. Lednev
Landulfo Silveira Jr
Publikationsdatum: 01.12.2023
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2023
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-023-03871-6

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Diagnosing COVID-19 in nasopharyngeal secretion through Raman spectroscopy: a feasibility study

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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