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01.01.2024 | Research

Differentially expressed miRNAs associated with generalized aggressive periodontitis

verfasst von: Esra Guzeldemir-Akcakanat, Deniz Sunnetci-Akkoyunlu, V. Merve Balta-Uysal, Tolgahan Özer, Elif Büşra Işik, Naci Cine

Erschienen in: Clinical Oral Investigations | Ausgabe 1/2024

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Abstract

Objective

This study aimed to investigate miRNA expression profiles in individuals with periodontitis which is a chronic inflammatory condition affecting the integrity of the periodontal attachment. miRNAs play a crucial role in gene regulation through various mechanisms, making them potential diagnostic markers and therapeutic targets for various diseases.

Materials and methods

A total of 25 individuals with aggressive periodontitis and 25 controls were included in the study. Gingival tissues were collected for miRNA isolation and cDNA synthesis. miRNAs associated with periodontitis, including hsa-miR-185-5p, hsa-miR-17, hs-miR-146a, hs-miR-146b, hs-miR-155, hs-miR-203, hs-miR-205, hs-miR-223, and hsa-miR-21-3p, were analyzed using a combination of miRTarBase database analysis and literature mining was performed. Real-time PCR was used to assess the expression patterns of the target miRNAs, and the data were analyzed using the REST program.

Results

The study revealed upregulated expression levels of hsa-miR-223-3p, hsa-miR-203b-5p, hsa-miR-146a-5p, hsa-miR-146b-5p, and hsa-miR-155-5p in individuals with periodontitis. Conversely, downregulated expression was observed for hsa-miR-185-5p, hsa-miR-21-3p, and hsa-miR-17-3p.

Conclusion

The findings suggest significant differences in the expression of specific miRNAs associated with inflammation in periodontitis. MZB1 acts as a hormone-regulated adipokine/pro-inflammatory cytokine, driving chronic inflammation and influencing cellular expansion. Predominantly expressed in marginal zone and B1 B cells, specialized subsets that respond rapidly to infections, MZB1 impacts immune protein synthesis and immune cell maturation, notably targeting microRNA-185 to potentially impede T cell development. Further research is needed to elucidate the functional significance and potential implications of these miRNAs.

Clinical relevance

miRNAs regulate the expression of target genes by finely tuning protein expression levels. The current findings provide compelling evidence of notable variations in the expression levels of specific miRNAs associated with inflammation in individuals affected by periodontitis; hence, miRNAs hold promise as potential therapeutic targets for periodontitis.

Ultracain^® DS 40mg/mL+6mcg/mL, Sanofi, Istanbul, Turkiye.

The Savant Bio 101 FastPrep ,Bio101, Vista, CA/Savant Instruments, Holbrook, NY, USA

The miRNeasy Tissue/Cells Advanced Mini Kit (217604), Qiagen^®, Düsseldorf, Germany

NanoDrop™ 2000/2000c Spectrophotometer, ThermoFisherScientific, NanoDropProducts, Wilmington, DE, USA.

miRCURY LNA RT Kit (339340), Qiagen^®, Düsseldorf, Germany.

miRCURY LNA miRNA PCR Assay, Qiagen^®, Düsseldorf, Germany.

miRCURY LNA miRNA SYBR^® Green PCR Kit, Qiagen^®, Düsseldorf, Germany.

LightCycler^® 480 System, RocheDiagnostics, Basel, Switzerland.

Relative Expression Software Tool (REST)https://www.gene-quantification.de/rest.html

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Titel: Differentially expressed miRNAs associated with generalized aggressive periodontitis
verfasst von: Esra Guzeldemir-Akcakanat
Deniz Sunnetci-Akkoyunlu
V. Merve Balta-Uysal
Tolgahan Özer
Elif Büşra Işik
Naci Cine
Publikationsdatum: 01.01.2024
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 1/2024
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-023-05404-5

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Differentially expressed miRNAs associated with generalized aggressive periodontitis