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02.03.2022 | Original Article

Effects of photobiomodulation therapy with red LED on inflammatory cells during the healing of skin burns

verfasst von: Thamyres Maria Silva Simões, José de Alencar Fernandes Neto, Cassiano Francisco Weege Nonaka, Maria Helena Chaves de Vasconcelos Catão

Erschienen in: Lasers in Medical Science | Ausgabe 7/2022

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Abstract

The aim of this study was to evaluate the effects of red light emitting diode (LED) photobiomodulation therapy protocol on inflammatory cells during the healing of third-degree skin burns. Fifty Wistar rats were randomly divided into control group (CTRL) (n = 25) and red group (RED) (n = 25), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, 28, and 32 days). Treatment animals were daily irradiated (630 nm ± 10 nm, 300 mW, 9 J/cm² per point, 30 s, continuous emission mode) at the 4 angles of the wound (total: 36 J/cm²). After specimen removal, histological sections were stained with hematoxylin and eosin for quantitative analysis of the inflammatory infiltrate (neutrophils and lymphocytes) under light microscopy. Greater number of inflammatory cells was observed in irradiated groups when compared to CTRL at 7, 14, 21, and 28 days, but with statistically significant difference only at 14 days (p = 0.02). At 32 days, higher inflammatory cell value was observed in CTRL when compared to RED, but with no statistically significant difference (p = 0.91). The results suggest that red LED, according to the protocol used, modulates the number of inflammatory cells in the early stages of the healing of third-degree skin burns. Nevertheless, this low-intensity light therapy may not, be efficient in reducing the number of neutrophils and lymphocytes in advanced stages of the repair process of skin burns. Further studies with other therapy protocols are needed to assess the effects of this type of light on the inflammatory response of skin burns.

World Health Organization (2018) Burns. https://www.who.int/news-room/fact-sheets/detail/burns. Accessed 12 de Jun 2020

Neves SMV, Nicolau RA, Filho AL, Mendes LM, Veloso AM (2014) Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats. Lasers Med Sci 29:2013–312. https://doi.org/10.1007/s10103-013-1312-7CrossRef

Catão MHCV, Nonaka CFW, Albuquerque RLC Jr, Bento PM, Oliveira RC (2015) Effects of red laser, infrared, photodynamic therapy, and green LED on the healing process of third-degree burns: clinical and histological study in rats. Lasers Med Sci 30:421–428. https://doi.org/10.1007/s10103-014-1687-0CrossRef

Ocon CA, Dos Santos SA, Caires JR et al (2019) Effects and parameters of the photobiomodulation in experimental models of third-degree burn: systematic review. Lasers Med Sci 34(3):637–648. https://doi.org/10.1007/s10103-018-2633-3CrossRefPubMed

Simões TMS, Fernandes Neto JA, de Oliveira TKB, Nonaka CFW, Catão MHCV (2020) Photobiomodulation of red and green lights in the repair process of third-degree skin burns. Lasers Med Sci 35(1):51–61. https://doi.org/10.1007/s10103-019-02776-7CrossRefPubMed

Hamblin MR (2017) Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophys 4(3):337–361. https://doi.org/10.3934/biophy.2017.3.337CrossRefPubMedPubMedCentral

Gabriel AF, Wagner VP, Correa C et al (2019) Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing. Lasers Med Sci 34(7):1465–1472. https://doi.org/10.1007/s10103-019-02745-0CrossRef

Fernandes Neto JA, Nonaka CFW, Catão MHCV (2019) Effect of blue LED on the healing process of third-degree skin burns: clinical and histological evaluation. Lasers Med Sci 34(4):721–728. https://doi.org/10.1007/s10103-018-2647-xCrossRef

Meyer TN, Silva AL (1999) A standard burn model using rats. Acta Cir Bras 14(4). https://doi.org/10.1590/S0102-86501999000400009

10.

Medeiros ML, Araújo-Filho I, da Silva EM et al (2017) Effect of low-level laser therapy on angiogenesis and matrix metalloproteinase-2 immunoexpression in wound repair. Lasers Med Sci 32(1):35–43. https://doi.org/10.1007/s10103-016-2080-yCrossRefPubMed

11.

Lee KC, Joory K, Moiemen NS (2014) History of burns: the past, present and the future. Burn Trauma 2(4):169–180. https://doi.org/10.4103/2321-3868.143620CrossRef

12.

Chaves ME, Araújo AR, Piancastelli AC et al (2014) Effects of low-power light therapy on wound healing: LASER × LED. An Bras Dermatol 89(4):616–623. https://doi.org/10.1590/abd1806-4841.20142519CrossRefPubMedPubMedCentral

13.

de Moraes JM, de Oliveira E, Mendonça D, Moura VB et al (2013) Anti-inflammatory effect of low-intensity laser on the healing of third-degree burn wounds in rats. Lasers Med Sci 28(4):1169–1176. https://doi.org/10.1007/s10103-012-1213-1CrossRefPubMed

14.

Ghaemi M, Sharifi D, Mokmeli S et al (2019) Comparison and evaluation of the low-level laser and the red and blue LED effects on wound healing in rabbit. J Lasers Med Sci 10(3):189‐193. https://doi.org/10.15171/jlms.2019.30

15.

de Sousa AP, de Aguiar Valença Neto Ade A, Marchionni AM et al (2011) Effect of LED phototherapy (λ700 ± 20 nm) on TGF-β expression during wound healing: an immunohistochemical study in a rodent model. Photomed Laser Surg 29(9):605–611CrossRef

16.

Oliveira Sampaio SC, de C Monteiro JS, Cangussú MC et al (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 28(3):799–806. https://doi.org/10.1007/s10103-012-1161-9CrossRefPubMed

17.

da Silva MS, Alves LP, Fernandes AB et al (2018) LED phototerapy in full-thickness burns induced by CO₂ laser in rats skin. Lasers Med Sci 33(7):1537–1547. https://doi.org/10.1007/s10103-018-2515-8CrossRef

18.

Frangež I, Nizič-Kos T, Frangež HB (2018) phototherapy with LED shows promising results in healing chronic wounds in diabetes mellitus patients: a prospective randomized double-blind study. Photomed Laser Surg 36(7):377–382. https://doi.org/10.1089/pho.2017.4382CrossRefPubMed

19.

Dall Agnol MA, Nicolau RA, de Lima CJ, Munin E (2009) Comparative analysis of coherent light action (laser) versus non-coherent light (light-emitting diode) for tissue repair in diabetic rats. Lasers Med Sci 24(6):909–916. https://doi.org/10.1007/s10103-009-0648-5CrossRefPubMed

20.

Leite SN, Andrade TA, Masson-Meyers S, Leite MN, Enwemeka CS, Frade MA (2014) Phototherapy promotes healing of cutaneous wounds in undernourished rats. An Bras Dermatol 89(6):899–904. https://doi.org/10.1590/abd1806-4841.20143356CrossRefPubMedPubMedCentral

21.

Catão MH, Costa RO, Nonaka CF, Albuquerque Junior RL, Costa IR (2016) Green LED light has anti-inflammatory effects on burns in rats. Burns 42(2):392–396. https://doi.org/10.1016/j.burns.2015.07.003CrossRefPubMed

22.

Silveira PCL, Ferreira KB, Rocha FR, Pieri BL, Pedroso GS, Souza CT et al (2016) Effect of low-power laser (LPL) and light-emitting diode (LED) on inflammatory response in burn wound healing. Inflammation 39:1395–1404. https://doi.org/10.1007/s10753-016-0371-xCrossRefPubMed

23.

Sousa AP, Paraguassú GM, Silveira NT, Souza J, Cangussú MC, Santos JN, Pinheiro AL (2013) Laser and LED phototherapies on angiogenesis. Lasers Med Sci 28:981–987. https://doi.org/10.1007/s10103-012-1187-zCrossRefPubMed

24.

Kim WS, Calderhead RG (2011) Is light-emitting diode phototherapy (LED-LLLT) really effective? Laser Therapy 20:205–215. https://doi.org/10.5978/islsm.20.205CrossRefPubMedPubMedCentral

Titel: Effects of photobiomodulation therapy with red LED on inflammatory cells during the healing of skin burns
verfasst von: Thamyres Maria Silva Simões
José de Alencar Fernandes Neto
Cassiano Francisco Weege Nonaka
Maria Helena Chaves de Vasconcelos Catão
Publikationsdatum: 02.03.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 7/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03537-9

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Effects of photobiomodulation therapy with red LED on inflammatory cells during the healing of skin burns

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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