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Japanese Journal of Ophthalmology

Erschienen in:

15.11.2023 | Laboratory Investigation

Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution

verfasst von: Tomoko Tsukahara-Kawamura, Nozomu Hanaoka, Eiichi Uchio

Erschienen in: Japanese Journal of Ophthalmology | Ausgabe 1/2024

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Abstract

Purpose

To investigate the virucidal effects of a polyvinyl alcohol iodine, Saniode, against 16 types of human mastadenovirus (HAdV) causing ophthalmic, respiratory, gastrointestinal, urinary, and systemic infections.

Study design

Laboratory investigation

Methods

Fifty microliters of Saniode were exposed to 10 μL each containing HAdV virus stock solution of 1 × 10⁶ copies/μL of HAdV-1, -2, -3, -4, 5, -6, -7, -8, -11, -37, -53, -54, -56, -64, -81, and -85 for 10 s, 30 s, 1 min, and 3 min. After neutralization with 0.5% sodium thiosulfate, the mixture was diluted by ten-fold serial dilution and inoculated into 24 wells containing confluent A549 cell monolayers. Virucidal effects were calculated relative to the positive control on days 7–10 and observed until 30 days post-infection.

Results

Saniode satisfied the EN-14476 criterion for virucidal effects (>99.99%) for all HAdV types at all exposure times, including at 10 s on days 7 to 10 post-infection. All types of HAdVs that reacted for > 1 min achieved 99.99% reduction, including after 30 days.

Conclusion

Saniode displayed virucidal effects against all tested HAdV types. Currently, with no specific medication available for HAdVs in ocular infection, this could be an option to prevent the spread of keratoconjunctivitis.

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Titel: Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution
verfasst von: Tomoko Tsukahara-Kawamura
Nozomu Hanaoka
Eiichi Uchio
Publikationsdatum: 15.11.2023
Verlag: Springer Japan
Erschienen in: Japanese Journal of Ophthalmology / Ausgabe 1/2024
Print ISSN: 0021-5155
Elektronische ISSN: 1613-2246
DOI: https://doi.org/10.1007/s10384-023-01027-1

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Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution

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