Erschienen in:
30.06.2023 | Sleep Breathing Physiology and Disorders • Original Article
The immune response to arterial damage in a mouse model of intermittent hypoxia: a transcriptomics analysis
verfasst von:
Chong Xu, Xiangyu Cheng, Xiaoting Wang, Weijun Huang, Yupu Liu, Haibo Ye, Jian Guan, Jinhong Shen, Hongliang Yi
Erschienen in:
Sleep and Breathing
|
Ausgabe 6/2023
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Abstract
Purpose
Mice can develop arterial damage and even atherosclerosis under intermittent hypoxia (IH); however, the specific mechanism of arterial damage induced by IH remains unclear. Hence, this research aimed to illustrate the underlying mechanism linking IH to arterial injury.
Materials and methods
The differential gene expression of the thoracic aorta under normoxia or IH mice was analyzed utilizing RNA sequencing. Furthermore, GO, KEGG pathway, and CIBERSORT analyses were carried out. For verification of the expression of candidate genes affected by IH, quantitative RT-qPCR (qRT-PCR) was conducted. Immunohistochemical (IHC) staining revealed immune cell infiltration in the thoracic aorta.
Results
The thickness of the intima-media of the mouse aorta was increased, and the fiber structure was disordered under IH. Transcriptomics analysis showed that in the aorta, 1137 upregulated genes and 707 downregulated genes were affected by IH, significantly related to the activation of the immune system and cell adhesion. Furthermore, B cell infiltration around the aorta was observed under IH.
Conclusions
IH might lead to structural changes in the aorta by activating the immune response and enhancing cell adhesion.