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01.12.2023 | Original Article

The pulse light mode enhances the effect of photobiomodulation on B16F10 melanoma cells through autophagy pathway

verfasst von: Zeqing Chen, Ruixiao Zhang, Haokuan Qin, Hui Jiang, Aixia Wang, Xiaolin Zhang, Shijie Huang, Miao Sun, Xuewei Fan, Zhicheng Lu, Yinghua Li, Shangfeng Liu, Muqing Liu

Erschienen in: Lasers in Medical Science | Ausgabe 1/2023

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Abstract

Photobiomodulation (PBM) is the use of low irradiance light of specific wavelengths to generate physiological changes and therapeutic effects. However, there are few studies on the effects of PBM of different LED light modes on cells. Here, we investigated the difference of influence between continuous wave (CW) and pulse-PBM on B16F10 melanoma cells. Our results suggested that the pulse mode had a more significant PBM than the CW mode on B16F10 melanoma cells. Our study confirmed that ROS and Ca²⁺ levels in B16F10 melanoma cells treated with pulse-PBM were significantly higher than those in the control and CW-PBM groups. One mechanism that causes the difference in CW and pulse-PBM action is that pulse-PBM activates autophagy of melanoma cells through the ROS/OPN3/Ca²⁺ signaling pathway, and excessive autophagy activation inhibits proliferation and apoptosis of melanoma cells. Autophagy may be one of the reasons for the difference between pulse- and CW-PBM on melanoma cells. More importantly, melanoma cells responded to brief PBM pulses by increasing intracellular Ca²⁺ levels.

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Titel: The pulse light mode enhances the effect of photobiomodulation on B16F10 melanoma cells through autophagy pathway
verfasst von: Zeqing Chen
Ruixiao Zhang
Haokuan Qin
Hui Jiang
Aixia Wang
Xiaolin Zhang
Shijie Huang
Miao Sun
Xuewei Fan
Zhicheng Lu
Yinghua Li
Shangfeng Liu
Muqing Liu
Publikationsdatum: 01.12.2023
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2023
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-023-03733-1

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The pulse light mode enhances the effect of photobiomodulation on B16F10 melanoma cells through autophagy pathway

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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