nach oben

Erschienen in:

17.08.2022

The synthetic opioid fentanyl increases HIV replication and chemokine co-receptor expression in vitro

verfasst von: Ling Kong, Mohamed Tarek M. Shata, Jennifer L. Brown, Michael S. Lyons, Kenneth E. Sherman, Jason T. Blackard

Erschienen in: Journal of NeuroVirology | Ausgabe 4-6/2022

Einloggen, um Zugang zu erhalten

Abstract

The US is experiencing a major public health crisis that is fueled by the illicit use of synthetic opioids including fentanyl. While several drugs of abuse can enhance viral replication and/or antagonize immune responses, the impact of specific synthetic opioids on HIV pathogenesis is poorly understood. Thus, we evaluated the effects of fentanyl on HIV replication in vitro. HIV-susceptible or HIV-expressing cell lines were incubated with fentanyl. HIV p24 synthesis and chemokine receptor levels were quantified by ELISA in culture supernatants and cell lysates, respectively. Addition of fentanyl resulted in a dose-dependent increase in HIV replication. Fentanyl enhanced expression of the HIV chemokine co-receptors CXCR4 and CCR5 and caused a dose-dependent decrease in cell viability. The opioid antagonist naltrexone blocked the effect of fentanyl on HIV replication and CCR5 receptor levels but not CXCR4 receptor levels. TLR9 expression was induced by HIV; however, fentanyl inhibited TLR9 expression in a dose-dependent manner. These data demonstrate that the synthetic opioid fentanyl can promote HIV replication in vitro. As increased HIV levels are associated with accelerated disease progression and higher likelihood of transmission, additional research is required to enhance the understanding of opioid-virus interactions and to develop new and/or optimized treatment strategies for persons with HIV and opioid use disorder.

Adachi A, Gendelman H, Koenig S, Folks T, Willey R, Rabson A, Martin M (1986) Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol 59:284–291CrossRef

Armenian P, Vo KT, Barr-Walker J, Lynch K (2017) Fentanyl, fentanyl analogs and novel synthetic opioids: a comprehensive review. Neuropharmacology 134:121–132CrossRef

Arnatt CK, Falls BA, Yuan Y, Raborg TJ, Masvekar RR, El-Hage N, Selley DE, Nicola AV, Knapp PE, Hauser KF, Zhang Y (2016) Exploration of bivalent ligands targeting putative mu opioid receptor and chemokine receptor CCR5 dimerization. Bioorg Med Chem 24:5969–5987CrossRef

Bagasra O, Pomerantz R (1993) Human immunodeficiency virus type 1 replication in peripheral blood mononuclear cells in the presence of cocaine. J Infect Dis 168:1157–1164CrossRef

Banerjee A, Strazza R, Wigdahl B, Pirrone V, Meucci O, Nonnemacher M (2011) Role of mu-opioid receptors as cofactors in human immunodeificiency virus type 1 disease progression and neuorpathogenesis. J Neurovirol 17:291–302CrossRef

Beltran JA, Pallur A, Chang SL (2006) HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Int Immunopharmacol 6:1459–1467CrossRef

Blackard JT, Brown JL, Lyons M (2019) Synthetic opioid use and common injection-associated viruses: expanding the translational research agenda. Curr HIV Res 17:94–101CrossRef

Blackard JT, Sherman KE (2021). Drugs of Abuse and Their Impact on Viral Pathogenesis. Viruses 13:2387

Bochud PY, Hersberger M, Taffé P, Bochud M, Stein CM, Rodrigues SD, Calandra T, Francioli P, Telenti A, Speck RF, Aderem A, Study SHC (2007) Polymorphisms in Toll-like receptor 9 influence the clinical course of HIV-1 infection. AIDS 21:441–446CrossRef

Browne EP (2020). The role of toll-like receptors in retroviral infection. Microorganisms 8.

Buitendijk M, Eszterhas SK, Howell AL (2014) Toll-like receptor agonists are potent inhibitors of human immunodeficiency virus-type 1 replication in peripheral blood mononuclear cells. AIDS Res Hum Retroviruses 30:457–467CrossRef

Börner C, Höllt V, Kraus J (2002) Involvement of activator protein-1 in transcriptional regulation of the human mu-opioid receptor gene. Mol Pharmacol 61:800–805CrossRef

Cai NS, Cadet JL (2008) The combination of methamphetamine and of the HIV protein, Tat, induces death of the human neuroblastoma cell line, SH-SY5Y. Synapse 62:551–552CrossRef

Caputi FF, Lattanzio F, Carretta D, Mercatelli D, Candeletti S, Romualdi P (2013) Morphine and fentanyl differently affect MOP and NOP gene expression in human neuroblastoma SH-SY5Y cells. J Mol Neurosci 51:532–538CrossRef

Celik F, Duran T (2019) Effects of fentanyl on pancreatic cancer cell proliferation and cancer stem cell differentiation. Cellular and Molecular Biology (Noisy-le-Grand, France) 65:21–25.

Cen P, Ye L, Su QJ, Wang X, Li JL, Lin XQ, Liang H, Ho WZ (2013) Methamphetamine inhibits Toll-like receptor 9-mediated anti-HIV activity in macrophages. AIDS Res Hum Retroviruses 29:1129–1137CrossRef

Chang SL, Beltran JA, Swarup S (2007) Expression of the mu opioid receptor in the human immunodeficiency virus type 1 transgenic rat model. J Virol 81:8406–8411CrossRef

Cranston K, Alpren C, John B, Dawson E, Roosevelt K, Burrage A, Bryant J, Switzer WM, Breen C, Peters PJ, Stiles T, Murray A, Fukuda HD, Adih W, Goldman L, Panneer N, Callis B, Campbell EM, Randall L, France AM, Klevens RM, Lyss S, Onofrey S, Agnew-Brune C, Goulart M, Jia H, Tumpney M, McClung P, Dasgupta S, Bixler D, Hampton K, Board A, Jaeger JL, Buchacz K Jr, DA, (2019) HIV diagnoses among persons who inject drugs - Northeastern Massachusetts, 2015–2018. Morb Mortal Wkly Rep 68:253–254CrossRef

Daniulaityte R, Juhascik MP, Strayer KE, Sizemore IE, Harshbarger KE, Antonides HM, Carlson R (2017) Overdose deaths related to fentanyl and its analogs - Ohio, January-February 2017. Morb Mortal Wkly Rep 66:904–908CrossRef

Dave RS, Ali H, Sil S, Knight LA, Pandey K, Madduri LSV, Qiu F, Ranga U, Buch S, Byrareddy SN (2020) NF-κB duplications in the promoter-variant HIV-1C LTR impact inflammation without altering viral replication in the context of simian human immunodeficiency viruses and opioid-exposure. Front Immunol 11:95CrossRef

De Minicis S, Candelaresi C, Marzioni M, Saccomano S, Roskams T, Casini A, Risaliti A, Salzano R, Cautero N, di Francesco F, Benedetti A, Svegliati-Baroni G (2008) Role of endogenous opioids in modulating HSC activity in vitro and liver fibrosis in vivo. Gut 57:352–364CrossRef

DeGruttolla V, Seage G, Mayer K, Horsburgh C (1989) Infectiousness of HIV between male homosexual partners. J Clin Epidemiol 42:849–856CrossRef

Delogu G, Moretti S, Antonucci A, Marandola M, Tellan G, Sale P, Carnevali R, Famularo G (2004a) Apoptogenic effect of fentanyl on freshly isolated peripheral blood lymphocytes. The Journal of Trauma: Injury, Infection, and Critical Care 57:75–81CrossRef

Delogu G, Moretti S, Antonucci A, Marandola M, Tellan G, Sale P, Carnevali R, Famularo G (2004b) Apoptogenic effect of fentanyl on freshly isolated peripheral blood lymphocytes. J Trauma 57:75–81CrossRef

Donadoni M, Huang W, Yarandi SS, Burdo TH, Chang SL, Sariyer IK (2021) Modulation of OPRM1 alternative splicing by morphine and HIV-1 Nef. J Neuroimmune Pharmacol. Epub ahead of print

Ebrahimkhani MR, Kiani S, Oakley F, Kendall T, Shariftabrizi A, Tavangar SM, Moezi L, Payabvash S, Karoon A, Hoseininik H, Mann DA, Moore KP, Mani AR, Dehpour A (2006) Naltrexone, an opioid receptor antagonist, attenuates liver fibrosis in bile duct ligated rats. Gut 55:1606–1616CrossRef

Ellis CR, Kruhlak NL, Kim MT, Hawkins EG, Stavitskaya L (2018) Predicting opioid receptor binding affinity of pharmacologically unclassified designer substances using molecular docking. PLoS ONE 13:e0197734CrossRef

Ellis RJ, Childers ME, Cherner M, Lazzaretto D, Letendre S, I; G, Group HNRC (2003) Increased human immunodeficiency virus loads in active methamphetamine users are explained by reduced effectiveness of antiretroviral therapy. J Infect Dis 188:1820–1826CrossRef

Folks TM, Clouse KA, Justement J, Rabson A, Duh E, Kehrl JH, Fauci AS (1989) Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone. Proc Natl Acad Sci U S A 86:2365–2368CrossRef

Guo CJ, Li Y, Tian S, Wang X, Douglas SD, Ho W (2002) Morphine enhances HIV infection of human blood mononuclear phagocytes through modulation of beta-chemokines and CCR5 receptor. J Investig Med 50:435–442CrossRef

He G, Li LI, Guan E, Chen J, Qin YI, Xie Y (2016) Fentanyl inhibits the progression of human gastric carcinoma MGC-803 cells by modulating NF-κB-dependent gene expression. Oncol Lett 12:563–571CrossRef

Hsu HY, Chang MH, Ni YH, Chen H (2004) Survey of hepatitis B surface variant infection in children 15 years after a nationwide vaccination programme in Taiwan. Gut 53:1499–1503CrossRef

Huang B, Wang H, Zheng Y, Li M, Kang G, Barreto-de-Souza V, Nassehi N, Knapp PE, Selley DE, Hauser KF, Zhang Y (2021) Structure-based design and development of chemical probes targeting putative MOR-CCR5 feterodimers to inhibit opioid exacerbated HIV-1 infectivity. J Med Chem 64:7702–7723CrossRef

Jaume M, Jacquet S, Cavaillès P, Macé G, Stephan L, Blanpied C, Demur C, Brousset P, Dietrich G (2004) Opioid receptor blockade reduces Fas-induced hepatitis in mice. Hepatology 40:1136–1143CrossRef

Jia K, Sun D, Ling S, Tian Y, Yang X, Sui J, Tang B, Wang L (2014) Activated δ-opioid receptors inhibit hydrogen peroxide-induced apoptosis in liver cancer cells through the PKC/ERK signaling pathwa. Mol Med Rep 10:839–847CrossRef

Jiang J, Wang M, Liang B, Shi Y, Su Q, Chen H, Huang J, Su J, Pan P, Li Y, Wang H, Chen R, Liu J, Zhao F, Ye L, Liang H (2016) In vivo effects of methamphetamine on HIV-1 replication: a population-based study. Drug Alcohol Depend 159:246–254CrossRef

Krishnan JM, Kong L, Blackard JT (2021). The synthetic opioid fentanyl increases HIV replication and chemokine co-receptor expression in an HIV indicator cell line. In: HIV and Liver Disease: Jackson Hole, WY.

Jones CM, Logan J, Gladden RM, Bohm MK (2015) Vital signs: demographic and substance use trends among heroin users - United States, 2002–2013. Morb Mortal Wkly Rep 64:719–725

Kanamori T, Togawa Iwata Y, Segawa H, Yamamuro T, Kuwayama K, Tsujikawa K, Inoue H (2018) Metabolism of fentanyl and acetylfentanyl in human-induced pluripotent stem cell-derived hepatocytes. Biological and Pharameutical Bulletin 41:106–114CrossRef

Kimpton J, Emerman M (1992) Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene. J Virol 66:2232–2239CrossRef

Kocak N, Ozen F, Yildirim IH, Duran Y (2017) Fentanyl inhibits tumorigenesis from human breast stem cells by inducing apoptosis. Asian Pac J Cancer Prev 18:735–739

Kong L, Karns R, Shata MTM, Brown JL, Lyons MS, Sherman KE, Blackard JT (2021) The synthetic opioid fentanyl enhances viral replication in vitro. PLoS ONE 16:e0249581CrossRef

Kraus J (2009) Regulation of mu-opioid receptors by cytokines. Front Biosci (schol Ed) 1:164–170CrossRef

Kraus J, Börner C, Giannini E, Höllt V (2003) The role of nuclear factor kappaB in tumor necrosis factor-regulated transcription of the human mu-opioid receptor gene. Mol Pharmacol 64:876–884CrossRef

Kraus J, Börner C, Lendeckel U, Höllt V (2006) Interferon-gamma down-regulates transcription of the mu-opioid receptor gene in neuronal and immune cells. J Neuroimmunol 181:13–18CrossRef

Li Y, Wang X, Tian S, Guo CJ, Douglas SD, Ho W (2002) Methadone enhances human immunodeficiency virus infection of human immune cells. J Infect Dis 185:118–122CrossRef

Li Y, Merrill JD, Mooney K, Song L, Wang X, Guo CJ, Savani RC, Metzger DS, Douglas SD, Ho WZ (2003) Morphine enhances HIV infection of neonatal macrophages. Pediatr Res 54:282–288CrossRef

Liang H, Wang X, Chen H, L S, Ye L, Wang SH, Wang YJ, Zhou L, Ho W (2008) Methamphetamine enhances HIV infection of macrophages. Am J Pathol 172:1617–1624CrossRef

Liao Y, Jiang J, Liang B, Wei F, Huang J, Pan P, Su J, Zhou B, Zang N, Ye L, Liang H (2017) Opiate use inhibits TLR9 signaling pathway in vivo: possible role in pathogenesis of HIV-1 infection. Sci Rep 7:13071CrossRef

Lipiński PFJ, Kosson P, Matalińska J, Roszkowski P, Czarnocki Z, Jarończyk M, Misicka A, Dobrowolski JC, Sadlej J (2019). Fentanyl family at the mu-opioid receptor: uniform assessment of binding and computational analysis. Molecules 24(4):740

Liu XZJ, Nishitani J, Chen H, Zack JA (2003) HIV-1 infection in peripheral blood lymphocytes (PBLs) exposed to alcohol. Virology 307:37–44CrossRef

Lu J, Liu Z, Zhao L, Tian H, Liu X, Yuan C (2013) In vivo and in vitro inhibition of human liver cancer progress by downregulation of the μ-opioid receptor and relevant mechanisms. Oncol Rep 30:1731–1738CrossRef

Ma H, Wang H, Li M, Barreto-de-Souza V, Reinecke BA, Gunta R, Zheng Y, Kang G, Nassehi N, Zhang H, An J, Selley DE, Hauser KF, Zhang Y (2020) Bivalent ligand aiming putative mu opioid receptor and chemokine receptor CXCR4 dimers in opioid enhanced HIV-1 entry. ACS Med Chem Lett 11:2318–2324CrossRef

Ma K, Ma P, Lu H, Liu S, Cao Q (2017) Fentanyl suppresses the survival of CD4+ T cells isolated from human umbilical cord blood through inhibition of IKKs-mediated NF-κB activation. Scand J Immunol 85:343–349CrossRef

Mattson CL, Tanz LJ, Quinn K, Karina M, Patel P, Davis NL (2021) Trends and geographic patterns in drug and synthetic opioid overdose deaths - United States, 2013–2019. Morb Mortal Wkly Rep 70:202–207CrossRef

Min BY, Kim NY, Jang ES, Shin CM, Lee SH, Park YS, Hwang JH, Jeong SH, Kim N, Lee DH, Kim J (2013) Ethanol potentiates hepatitis B virus replication through oxidative stress-dependent and -independent transcriptional activation. Biochem Biophys Res Commun 431:92–97CrossRef

Miyagi T, Chuang LF, Doi RH, Carlos MP, Torres JV, Chuang RY (2000) Morphine induces gene expression of CCR5 in human CEMx174 lymphocytes. J Biol Chem 275:31305–10.

Moallef S, Nosova E, Nolan S, Fairbairn N, Loh J, Hayashi K, Milloy MJ (2021) Prevalence and longitudinal correlates of recent exposure to fentanyl among HIV-positive people who use unregulated drugs during a community-wide overdose crisis. AIDS Care 33:1560–1568CrossRef

Nair MP, Chadha KC, Hewitt RG, Mahajan S, Sweet A, Schwartz S (2000) Cocaine differentially modulates chemokine production by mononuclear cells from normal donors and human immunodeficiency virus type 1-infected patients. Clin Vaccine Immunol 7:96–100

Nair MP, Saiyed Z (2011) Effect of methamphetamine on expression of HIV coreceptors and CC-chemokines by dendritic cells. Life Sci 88:987–994CrossRef

Nair MP, Saiyed ZM, Nair N, Gandhi NH, Rodriguez JW, Boukli N, Provencio-Vasquez E, Malow RM, Miguez-Burbano M (2009) Methamphetamine enhances HIV-1 infectivity in monocyte derived dendritic cells. J Neuroimmune Pharmacol 4:129–139CrossRef

Napuri J, Pilakka-Kanthikeel S, Raymond A, Agudelo M, Yndart-Arias A, Saxena SK, Nair M (2013) Cocaine enhances HIV-1 infectivity in monocyte derived dendritic cells by suppressing microRNA-155. PLoS ONE 8:e83682CrossRef

O’Donnell J, Gladden RM, Goldberger BA, Mattson CL, Kariisa M (2020) Notes from the field: opioid-involved overdose deaths with fentanyl or fentanyl analogs detected - 28 states and the District of Columbia, July 2016-December 2018. Morb Mortal Wkly Rep 69:271–273CrossRef

O’Donnell JK, Gladden RM, Seth P (2017) Trends in deaths involving heroin and synthetic opioids excluding methadone, and law enforcement drug product reports, by census region - United States, 2006–2015. Morb Mortal Wkly Rep 66:897–903CrossRef

Offersen R, Nissen SK, Rasmussen TA, Østergaard L, Denton PW, Søgaard OS, Tolstrup M (2016) A novel toll-like receptor 9 agonist, MGN1703, enhances HIV-1 transcription and NK cell-mediated inhibition of HIV-1-infected autologous CD4+ T cells. J Virol 90:4441–4453CrossRef

Peterson PK, Gekker G, Chao CC, Schut R, Molitor TW Jr, HB (1991) Cocaine potentiates HIV-1 replication in human peripheral blood mononuclear cell cocultures. Involvement of transforming growth factor-beta. J Immunol 146:81–84

Peterson PK, Sharp BM, Gekker G, Portoghese PS, Sannerud K Jr, HB (1990) Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures. AIDS 4:869–873CrossRef

Platt EJ, Wehrly K, Kuhmann SE, Chesebro B, Kabat D (1998) Effects of CCR5 and CD4 cell surface concentrations on infections by macrophage tropic isolates of human immunodeficiency virus type 1. J Virol 72:2855–2864CrossRef

Popovic M, Sarngadharan MG, Read E, Gallo RC (1984) Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500CrossRef

Qi L, Gang L, Hang KW, Ling CH, Xiaofeng Z, Zhen L, Wai YD, Sang PW (2011) Programmed neuronal cell death induced by HIV-1 tat and methamphetamine. Microsc Res Tech 74:1139–1144CrossRef

Qin Y, Li L, Chen J, Tang X, Liao C, Xie Y, Xiao Q (2012) Fentanyl inhibits progression of human gastric cancer MGC-803 cells by NF-kB down regulation and PTEN up regulation in vitro. Oncol Res 20:61–69CrossRef

Roth MD, Whittaker KM, Choi R, Tashkin DP, Baldwin GC (2005) Cocaine and sigma-1 receptors modulate HIV infection, chemokine receptors, and the HPA axis in the huPBL-SCID model. J Leukoc Biol 78:1198–1203CrossRef

Rudd RA, Aleshire N, Zibbell JE, Gladden R (2016a) Increases in drug and opioid overdose deaths–United States, 2000–2014. Morb Mortal Wkly Rep 64:1378–1382CrossRef

Rudd RA, Seth P, David F, Scholl L (2016b) Increases in drug and opioid-involved overdose deaths - United States, 2010–2015. Morb Mortal Wkly Rep 65:1445–1452CrossRef

Sahu G, Farley K, El-Hage N, Aiamkitsumrit B, Fassnacht R, Kashanchi F, Ochem A, Simon GL, Karn J, Hauser KF, Tyagi M (2015) Cocaine promotes both initiation and elongation phase of HIV-1 transcription by activating NF-κB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR. Virology 483:185–202CrossRef

Scheidegger C, Zimmerli W (1989) Infectious complications in drug addicts: seven-year review of 269 hospitalized narcotics abusers in Switzerland. Rev Infect Dis 11:486–493CrossRef

Somerville NJ, O’Donnell J, Gladden RM, Zibbell JE, Green TC, Younkin M, Ruiz S, Babakhanlou-Chase H, Chan M, Callis BP, Kuramoto-Crawford J, Nields HM, Walley A (2017) Characteristics of fentanyl overdose - Massachusetts, 2014–2016. Morb Mortal Wkly Rep 66:382–386CrossRef

Springer YP, Gladden RM, O’Donnell J, Seth P (2019) Notes from the field: fentanyl drug submissions - United States, 2010–2017. Morb Mortal Wkly Rep 68:41–43CrossRef

Squinto SP, Mondal D, Block AL, Prakash O (1990) Morphine-induced transactivation of HIV-1 LTR in human neuroblastoma cells. AIDS Res Hum Retroviruses 6:1163–1168CrossRef

Steele AD, Henderson EE, Rogers T (2003) Mu-opioid modulation of HIV-1 coreceptor expression and HIV-1 replication. Virology 309:99–107CrossRef

Steele AD, Szabo I, Bednar F, Rogers T (2002) Interactions between opioid and chemokine receptors: heterologous desensitization. Cytokine Growth Factor Review 13:209–222CrossRef

Swepson C, Ranjan A, Balasubramaniam M, Pandhare J, Dash C (2016) Cocaine enhances HIV-1 transcription in macrophages by inducing p38 MAPK phosphorylation. Front Microbiol 7:823CrossRef

Sánchez-Carbonell X, Vilaregut A (2001) A 10-year follow-up study on the health status of heroin addicts based on official registers. Addiction 96:1777–1786CrossRef

Tahamtan A, Tavakoli-Yaraki M, Mokhtari-Azad T, Teymoori-Rad M, Bont L, Shokri F, Salimi V (2016) Opioids and viral infections: a double-edged sword. Front Microbiol 7:970CrossRef

Talu A, Rajaleid K, Abel-Ollo K, Rüütel K, Rahu M, Rhodes T, Platt L, Bobrova N, Uusküla A (2010) HIV infection and risk behaviour of primary fentanyl and amphetamine injectors in Tallinn, Estonia: Implications for intervention. International Journal of Drug Policy 21:56–63CrossRef

Tang B, Li Y, Yuan S, Tomlinson S, He S (2013) Upregulation of the δ opioid receptor in liver cancer promotes liver cancer progression both in vitro and in vivo. Int J Oncol 43:1281–1290CrossRef

Tang B, Zhang Y, Liang R, Yuan P, Du J, Wang H, Wang L (2011) Activation of the δ-opioid receptor inhibits serum deprivation-induced apoptosis of human liver cells via the activation of PKC and the mitochondrial pathway. Int J Mol Med 28:1077–1085

Tiscione NB, Wegner K (2017) Validation of the Neogen® fentanyl ELISA kit for blood and urine. J Anal Toxicol 41:313–317

Turchan-Cholewo J, Dimayuga FO, Ding Q, Keller JN, Hauser KF, Knapp PE, Bruce-Keller AJ (2008) Cell-specific actions of HIV-Tat and morphine on opioid receptor expression in glia. J Neurosci Res 86:2100–2110CrossRef

Tyagi M, Bukrinsky M, Simon G (2016) Mechanisms of HIV transcriptional regulation by drugs of abuse. Curr HIV Res 14:442–454CrossRef

Vesanen M, Wessman M, Salminen M, Vaheri A (1992) Activation of integrated human immunodeficiency virus type 1 in human neuroblastoma cells by the cytokines tumour necrosis factor alpha and interleukin-6. J Gen Virol 73(Pt 7):1753–1760CrossRef

Vibholm LK, Konrad CV, Schleimann MH, Frattari G, Winckelmann A, Klastrup V, Jensen NM, Jensen SS, Schmidt M, Wittig B, Zuwala K, Mack K, Olesen R, Hua S, Lichterfeld M, Østergaard L, Denton PW, Tolstrup M, Søgaard OS (2019) Effects of 24-week Toll-like receptor 9 agonist treatment in HIV type 1+ individuals. AIDS 33:1315–1325CrossRef

Wang X, Ma TC, Li JL, Zhou Y, Geller EB, Adler MW, Peng JS, Zhou W, Zhou DJ, Ho W (2015) Heroin inhibits HIV-restriction miRNAs and enhances HIV infection of macrophages. Front Microbiol 6:1230CrossRef

Wang XDS, Metzger DS, Guo CJ, Li Y, O’Brien CP, Song L, Davis-Vogal A, Ho WZ (2002) Alcohol potentiates HIV-1 infection of human blood mononuclear phagocytes. Alcohol Clinical and Experimental Research 26:1880–1886CrossRef

Wang Y, Chen M (2017) Fentanyl ameliorates severe acute pancreatitis-induced myocardial injury in rats by regulating NF-κB signaling pathway. Med Sci Monit 23:3276–3283CrossRef

Wang Y, Wang X, Ye L, Li J, Song L, Fulambarkar N, Ho W (2012) Morphine suppresses IFN signaling pathway and enhances AIDS virus infection. PLoS ONE 7:e31167CrossRef

Wu F, Slawson MH, Johnson-Davis K (2017) Metabolic patterns of fentanyl, meperidine, methylphenidate, tapentadol and tramadol observed in urine, serum or plasma. J Anal Toxicol 41:289–299

Zadina JE, Harrison LM, Ge LJ, Kastin J, Chang S (1994) Differential regulation of mu and delta opiate receptors by morphine, selective agonists and antagonists and differentiating agents in SH-SY5Y human neuroblastoma cells. J Pharmacol Exp Ther 270:1086–1096

Zadina JE, Chang SL, Ge LJ, Kastin AJ (1993) Mu opiate receptor down-regulation by morphine and up-regulation by naloxone in SH-SY5Y human neuroblastoma cells. J Pharmacol Exp Ther 265:254–262

Zhang L, Belkowski JS, Briscoe T, Rogers TJ (2012) Regulation of mu opioid receptor expression in developing T cells. J Neuroimmune Pharmacol 7:835–842CrossRef

Zhang S, Alexander L, Wang T, Agler M, Zhou N, Fang H, Kadow J, Clapham P, Lin PF (2010) Protection against HIV-envelope-induced neuronal cell destruction by HIV attachment inhibitors. Arch Virol 155:777–781CrossRef

Zhang XL, Chen ML, Zhou SL (2014) Fentanyl increases colorectal carcinoma cell apoptosis by inhibition of NF-κB in a Sirt1-dependent manner. Asian Pac J Cancer Prev 15:10015–10020CrossRef

Zhu JW, Liu FL, Mu D, Deng DY, Zheng YT (2017) Heroin use is associated with lower levels of restriction factors and type I interferon expression and facilitates HIV-1 replication. Microbes Infect 19:288–294CrossRef

Titel: The synthetic opioid fentanyl increases HIV replication and chemokine co-receptor expression in vitro
verfasst von: Ling Kong
Mohamed Tarek M. Shata
Jennifer L. Brown
Michael S. Lyons
Kenneth E. Sherman
Jason T. Blackard
Publikationsdatum: 17.08.2022
Verlag: Springer International Publishing
Erschienen in: Journal of NeuroVirology / Ausgabe 4-6/2022
Print ISSN: 1355-0284
Elektronische ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-022-01090-3

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Nicht Creutzfeldt Jakob, sondern Abführtee-Vergiftung

29.05.2024 Hyponatriämie Nachrichten

Eine ältere Frau trinkt regelmäßig Sennesblättertee gegen ihre Verstopfung. Der scheint plötzlich gut zu wirken. Auf Durchfall und Erbrechen folgt allerdings eine Hyponatriämie. Nach deren Korrektur kommt es plötzlich zu progredienten Kognitions- und Verhaltensstörungen.

Schutz der Synapsen bei Alzheimer

29.05.2024 Morbus Alzheimer Nachrichten

Mit einem Neurotrophin-Rezeptor-Modulator lässt sich möglicherweise eine bestehende Alzheimerdemenz etwas abschwächen: Erste Phase-2-Daten deuten auf einen verbesserten Synapsenschutz.

Sozialer Aufstieg verringert Demenzgefahr

24.05.2024 Demenz Nachrichten

Ein hohes soziales Niveau ist mit die beste Versicherung gegen eine Demenz. Noch geringer ist das Demenzrisiko für Menschen, die sozial aufsteigen: Sie gewinnen fast zwei demenzfreie Lebensjahre. Umgekehrt steigt die Demenzgefahr beim sozialen Abstieg.

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4-6/2022

A pilot study of cognition and creativity among persons with HIV disease referred for neuropsychological evaluation

Response to “a fatal case of Guillain-Barré syndrome after infection with COVID-19”

Systematic post-mortem analysis of brain tissue from an HIV-1 subtype C viremic decedent revealed a paucity of infection and pathology

Cognitive performance in a South African cohort of people with HIV and comorbid major depressive disorder

The evaluation expression of non-coding RNAs in response to HSV-G47∆ oncolytic virus infection in glioblastoma multiforme cancer stem cells