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Japanese Journal of Ophthalmology

Erschienen in:

05.02.2024 | Laboratory Investigation

The existence of senescent cells in conjunctival epithelium from elderly individuals

verfasst von: Yasufumi Tomioka, Koji Kitazawa, Kohsaku Numa, Jun-Wei B. Hughes, Norihiko Yokoi, Chie Sotozono

Erschienen in: Japanese Journal of Ophthalmology | Ausgabe 2/2024

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Abstract

Purpose

The ocular surface microenvironment changes with aging. However, it remains unclear if cellular senescence influences the ocular surface. We investigated the presence of p16^INK4a-expressing senescent cells in healthy human conjunctiva.

Study design

Clinical and experimental.

Methods

Healthy conjunctival tissue samples were obtained from middle-aged and elderly subjects. RT-qPCR was performed to assess the expression of senescence markers CDKN2A (p16^INK4a) and CDKN1A (p21^CIP1/WAF1) and immunostaining was performed to examine the expression of the senescence marker p16^INK4a, stem cell markers Ki67 and p63, tight-junction marker ZO-1.

Results

Our study involved 19 conjunctival tissue samples (10 elderly and 9 middle-aged), mean age [elderly: 75.8 ± 3.7 years (72–81), middle-aged: 52.7 ± 7 years (38–59)], sex (elderly: 3 men, 7 women; middle-aged: 3 men, 6 women). The expression of p16^INK4a was significantly increased at the RNA level in the elderly compared to middle-aged (p < 0.05). Positivity rate of p16^INK4a was significantly elevated in the elderly (15.0 ± 7.8%) compared to middle-aged (0.2 ± 0.6%) (p < 0.05). Positivity rate of Ki67and p63 was significantly reduced in the elderly (1.7 ± 1.7% and 16.5 ± 9.5%) compared to middle-aged (3.9 ± 1.8% and 24.7 ± 5.7%) (p < 0.05). ZO-1 expression was reduced in tissue samples showing p16^INK4a-positivity but retained in tissue samples in which p16^INK4a was undetectable.

Conclusions

Senescent cells accumulate with age in the conjunctival epithelium, accompanied by a decrease in Ki67, p63 and ZO-1 expressing cells.

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Titel: The existence of senescent cells in conjunctival epithelium from elderly individuals
verfasst von: Yasufumi Tomioka
Koji Kitazawa
Kohsaku Numa
Jun-Wei B. Hughes
Norihiko Yokoi
Chie Sotozono
Publikationsdatum: 05.02.2024
Verlag: Springer Japan
Erschienen in: Japanese Journal of Ophthalmology / Ausgabe 2/2024
Print ISSN: 0021-5155
Elektronische ISSN: 1613-2246
DOI: https://doi.org/10.1007/s10384-023-01047-x

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The existence of senescent cells in conjunctival epithelium from elderly individuals

Abstract

Purpose

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Results

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